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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene continues to be observed in several tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical position in marketing mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been explained. Two extra splice variants were being documented but detailed nucleotide sequence facts just isn't nonetheless offered.

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Blocking peptides are peptides that bind particularly on the concentrate on antibody and block antibody binding. These peptide generally has the epitope regarded through the antibody.

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This tutorial outlines even more achievable optimization of cellular and immunoassay parameters to ensure the very best benefits are...

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This tutorial outlines further doable optimization of mobile and immunoassay parameters to be sure the best possible final results are...

Antibodies sure to the blocking peptide now not bind for the epitope to the focus on protein. By evaluating the staining within the blocked antibody versus the antibody on your own, one can see which staining is specific.

Target Information and facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins operate website as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E forms a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by means of S phase.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...

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